Liquid-crystal-television-based optical-digital processor for measurement of shortening velocity in single rat heart cells

Rao V. Yelamarty; Francis T. S. Yu; Russell L. Moore; Joseph Y. Cheung

doi:10.1117/12.47793

1 April 1991 Liquid-crystal-television-based optical-digital processor for measurement of shortening velocity in single rat heart cells

Rao V. Yelamarty, Francis T. S. Yu, Russell L. Moore, Joseph Y. Cheung

Author Affiliations +

Proceedings Volume 1398, CAN-AM Eastern '90; (1991) https://doi.org/10.1117/12.47793
Event: CAN-AM Eastern '90: 1st Canadian-American Eastern Regional Conference on Applications of Optical Engineering, 1990, Rochester, NY, United States

Abstract

Cardiac cells exhibit rhythmic contractions when electrically paced and maintain striation patterns throughout the contraction-relaxation cycle similar to one-dimensional diffraction grating that changes in spatial frequency. The current study demonstrates the application of an on-line hybrid optical-digital processor with liquid crystal television (LCTV) acting as a spatial light modulator(SLM) to monitor the dynamics of contraction of single cardiac cells in real-time . The processor which is interfaced with a phase contrast microscope performs the Fourier transformation of the cell''s striated image optically and records the Fourier spectra digitally using a charge coupled device (CCD) camera. A series of digitized images of the Fourier spectra each of duration of 16 . 67 msec (non-interlaced frames) is captured during one contraction cycle (''4000 msec). The sarcomere length at each time point and contraction velocities are calculated. The overall system is initially calibrated using a high-resolution Ealing target. Experimental results demonstrate the improvement of contrast by about 300 using the LCTV and the optical-digital processor can faithfully track sarcomere mechanics. 1 .

Citation Download Citation

Rao V. Yelamarty, Francis T. S. Yu, Russell L. Moore, and Joseph Y. Cheung "Liquid-crystal-television-based optical-digital processor for measurement of shortening velocity in single rat heart cells", Proc. SPIE 1398, CAN-AM Eastern '90, (1 April 1991); https://doi.org/10.1117/12.47793

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