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In previous investigations it was found that prostaglanding E1 (PGE1) and Bradiquinine (B), liberated in the inflammatory process, produced a significant increment on the Plasma fibrition Level (PFL), indicative of inflammatory process. The mentioned increment was completely abolished by the irradiation with HeNe laser in the area of infection of the mentioned substances. In the current investigation it was studied the effect of HeNe laser radiation on the P.F.L. of rats injected with another substance related with the inflammatory process and tissular injury: Thromboxane (Tx). It is though that the signal to increase P.F.L. is though an adrenal and an extra adrenal pathways. To study it we injected normal and medullectomized animals and both showed marked increment of P.F.L. Then we repeated the experiment but followed immediately by HeNe laser radiation and we noted a complete blockage of the P.F.L. increment in both groups which suggest that the effect is extra-adrenal. All substances were injected I.M. once daily X 3 days. Immediately after injection the area was irradiated with HeNe laser, 1.5 J total energy. In the normal, non injected non irradiated animals the P.F.L. reached 210.3 + 1.15 mg%. The single injection of Tx did not modify the P.F.L. compared with the previous group. The HeNe irradiation alone did not modify the P.F.L. in the animals injected with Tx only. The animals injected with PGE1+B showed a marked increment of P.F.L. to 337.6 + 14.5 mg%; the HeNe laser radiation completely abolished the increment (231 + 22.3 mg%). But in the animals injected with PGE1+B+Tx, the P.F.L. reached even larger values: 375.2 + 15.3 mg%. The HeNe laser radiation produced a partial blockage in P.F.L. increment (270.3 +/- 13.4 mg%). Showing a significant difference (p < 0.001) compared with normal rats or with rats injected with PGE1+B+Tx without radiation. In conclusion Tx potentiate the effect of PGE+B on the P.F.L. The HeNe laser blocks completely the interaction of PGE1+B on the P.F.L. but only partially the interaction PGE(subscript 1+B+Tx.
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