Single-color laser-induced fluorescence detection and capillary gel electrophoresis for DNA sequencing

Da Yong Chen; Harold Swerdlow; Heather Harke; Jian Zhong Zhang; Norman J. Dovichi

doi:10.1117/12.44240

1 July 1991 Single-color laser-induced fluorescence detection and capillary gel electrophoresis for DNA sequencing

Da Yong Chen, Harold Swerdlow, Heather Harke, Jian Zhong Zhang, Norman J. Dovichi

Proceedings Volume 1435, Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications; (1991) https://doi.org/10.1117/12.44240
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

Low zeptomole (1 zmol equals 10-21 mol = 600 molecules) detection limits are produced for DNA sequencing by capillary gel electrophoresis. A 750 (mu) w green helium-neon laser ((lambda) equals 543.5 nm) is used to excite tetramethylrhodamine-labeled DNA fragments in a sheath-flow cuvette. A cooled photomultiplier tube is used to detect fluorescence in a single spectral channel. Sequencing data is generated at a rate of about 70 bases/hour.

Citation Download Citation

Da Yong Chen, Harold Swerdlow, Heather Harke, Jian Zhong Zhang, and Norman J. Dovichi "Single-color laser-induced fluorescence detection and capillary gel electrophoresis for DNA sequencing", Proc. SPIE 1435, Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications, (1 July 1991); https://doi.org/10.1117/12.44240

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