Scanning force microscopy of circular DNA and chromatin in air and propanol

James Vesenka; Helen G. Hansma; Cristina Siegerist; Giuliano Siligardi; Eric Schabtach; Carlos J. Bustamante

doi:10.1117/12.58189

1 May 1992 Scanning force microscopy of circular DNA and chromatin in air and propanol

James Vesenka, Helen G. Hansma, Cristina Siegerist, Giuliano Siligardi, Eric Schabtach, Carlos J. Bustamante

Proceedings Volume 1639, Scanning Probe Microscopies; (1992) https://doi.org/10.1117/12.58189
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

High resolution electron micrographs of microtips show nanometer surface irregularities. We propose that the lower contact forces, and consequent greater sensitivity, from imaging in propanol enables small biomolecules to be imaged at higher resolution by these 'nanotips'. However, larger molecules, such as the nucleosomes of chromatin, are still imaged by the bulk of the tip and no improvement of resolution is obtained.

Citation Download Citation

James Vesenka, Helen G. Hansma, Cristina Siegerist, Giuliano Siligardi, Eric Schabtach, and Carlos J. Bustamante "Scanning force microscopy of circular DNA and chromatin in air and propanol", Proc. SPIE 1639, Scanning Probe Microscopies, (1 May 1992); https://doi.org/10.1117/12.58189

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