Three-dimensional structure of neurons from peripheral autonomic ganglia using confocal microscope images

Steven M. Miller; Philip Schmalz; Leonid Ermilov; James Tarara; Ruchun Ma; James Brewer; Joseph H. Szurszewski

doi:10.1117/12.131116

22 September 1992 Three-dimensional structure of neurons from peripheral autonomic ganglia using confocal microscope images

Steven M. Miller, Philip Schmalz, Leonid Ermilov, James Tarara, Ruchun Ma, James Brewer, Joseph H. Szurszewski

Author Affiliations +

Proceedings Volume 1808, Visualization in Biomedical Computing '92; (1992) https://doi.org/10.1117/12.131116
Event: Visualization in Biomedical Computing, 1992, Chapel Hill, NC, United States

Abstract

An understanding of neuronal physiology at the single unit level requires knowledge of the functional properties of the neurons as well as a detailed description of its morphology. A full morphological description should include a three-dimensional (3-D) analysis. In this paper, we describe for the first time, 3-D morphology of individual neurons of the peripheral autonomic nervous system intact within whole-mount preparations. Neurons were filled with the fluorescent dye Lucifer Yellow and imaged by laser scanning confocal microscopy. Three- dimensional reconstructions were obtained using volume rendering methods on a set of serial optical sections obtained for each neuron. The 3-D images of the neurons we studied showed a complexity of shape and detail that is not readily apparent from images viewed by traditional microscopy.

Citation Download Citation

Steven M. Miller, Philip Schmalz, Leonid Ermilov, James Tarara, Ruchun Ma, James Brewer, and Joseph H. Szurszewski "Three-dimensional structure of neurons from peripheral autonomic ganglia using confocal microscope images", Proc. SPIE 1808, Visualization in Biomedical Computing '92, (22 September 1992); https://doi.org/10.1117/12.131116

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