Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

K. Bruce Jacobson; Heinrich F. Arlinghaus; Mitchel J. Doktycz; R. A. Sachleben; Gilbert M. Brown; F. W. Larimer

doi:10.1117/12.146716

24 June 1993 Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

K. Bruce Jacobson, Heinrich F. Arlinghaus, Mitchel J. Doktycz, R. A. Sachleben, Gilbert M. Brown, F. W. Larimer

Author Affiliations +

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146716
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.

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K. Bruce Jacobson, Heinrich F. Arlinghaus, Mitchel J. Doktycz, R. A. Sachleben, Gilbert M. Brown, and F. W. Larimer "Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146716

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