Crystallographic phase retrieval through image processing under constraints

Kam Y. J. Zhang

doi:10.1117/12.161989

9 November 1993 Crystallographic phase retrieval through image processing under constraints

Kam Y. J. Zhang

Proceedings Volume 2029, Digital Image Recovery and Synthesis II; (1993) https://doi.org/10.1117/12.161989
Event: SPIE's 1993 International Symposium on Optics, Imaging, and Instrumentation, 1993, San Diego, CA, United States

Abstract

The crystallographic image processing techniques of Sayre's equation, molecular averaging, solvent flattening and histogram matching are combined in an integrated procedure for macromolecular phase retrieval. It employs the constraints of the local shape of electron density, equal molecules, solvent flatness and correct electron density distribution. These constraints on electron density image are satisfied simultaneously by solving a system of non- linear equations using fast Fourier transform. The electron density image is further filtered under the constraint of observed diffraction amplitudes. The effect of each constraint on phase retrieval is examined. The constraints are found to work synergistically in phase retrieval. Test results on 2Zn insulin are presented.

Citation Download Citation

Kam Y. J. Zhang "Crystallographic phase retrieval through image processing under constraints", Proc. SPIE 2029, Digital Image Recovery and Synthesis II, (9 November 1993); https://doi.org/10.1117/12.161989

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available