Raman spectrosopic characterization of human malignant tissues: implications for a percutaneous optical biopsy technique for in-situ tissue diagnosis

Douglas C. B. Redd M.D.; Christopher J. Frank; Zhe Chuan Feng; Ted S. Gansler; Richard L. McCreery

doi:10.1117/12.166824

15 January 1994 Raman spectrosopic characterization of human malignant tissues: implications for a percutaneous optical biopsy technique for in-situ tissue diagnosis

Douglas C. B. Redd M.D., Christopher J. Frank, Zhe Chuan Feng, Ted S. Gansler, Richard L. McCreery

Author Affiliations +

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166824
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Recent advancements in the technique of Raman spectroscopy now make it possible to achieve rapid, minimally invasive and non-destructive characterization of tissues. In order to evaluate the efficacy of this technique for diagnosis, the Raman spectra of normal and neoplastic human tissues (e.g., breast, kidney, liver and colon) were obtained utilizing visible and near-IR excitation. Normal breast tissue and colon adenocarcinoma showed major Raman features due to the presence of carotenoids and lipids. In breast carcinoma, the features due to lipids were attenuated and as fibrosis (desmoplasia) increased, new spectral features attributable to collagen were observed. Samples of normal and neoplastic liver and kidney show unique spectral differences sufficient to permit tissue differentiation.

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Douglas C. B. Redd M.D., Christopher J. Frank, Zhe Chuan Feng, Ted S. Gansler, and Richard L. McCreery "Raman spectrosopic characterization of human malignant tissues: implications for a percutaneous optical biopsy technique for in-situ tissue diagnosis", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166824

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