Intracellular relocalization of anionic photosensitizers measured by cw and time-gated microscopy and spectroscopy

Angelika C. Rueck; Herbert Schneckenburger; Wolfgang S. L. Strauss; Michael H. Gschwend; Rudolf W. Steiner

doi:10.1117/12.200888

1 February 1995 Intracellular relocalization of anionic photosensitizers measured by cw and time-gated microscopy and spectroscopy

Angelika C. Rueck, Herbert Schneckenburger, Wolfgang S. L. Strauss, Michael H. Gschwend, Rudolf W. Steiner

Author Affiliations +

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200888
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Intracellular modifications of anionic photosensitizers during PDT were observed, dependent on the growth phase of the incubated cells. In addition to steady-state spectroscopy, time- resolved fluorescence spectra between different time gates were measured. Comparative results were obtained for the sensitizers meso-tetra(4-sulfonatophenyl)porphyrine (TPPS₄) and aluminumsulfophthalocyanine (AlS_2-3Pc). In both cases relocalization of the sensitizer could be observed exclusively for cells in the growing phase, whereas no relocalization was detected for cells in the stationary phase. Moreover, time-gated spectra were different for the two cell types. The formation of a fluorescent band around 615 nm during PDT for the sensitizer TPPS₄ was observed mainly for cells in the growing phase. This species was correlated with a short fluorescence decay time (< 5 ns). Similar observations were done with AlS_2-3Pc. A fluorescence band blue-shifted in the same order of magnitude (about 30 nm from the original band) and measured predominantly between an `early' time-gate (0-5 ns) was detected for cells in the growing phase.

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Angelika C. Rueck, Herbert Schneckenburger, Wolfgang S. L. Strauss, Michael H. Gschwend, and Rudolf W. Steiner "Intracellular relocalization of anionic photosensitizers measured by cw and time-gated microscopy and spectroscopy", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200888

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