Distribution of TCDD in blood constituents of rats and humans

J. M. Wirsing; L. W.D. Weber; A. A. Kettrup; Karl K. Rozman

doi:10.1117/12.224098

9 October 1995 Distribution of TCDD in blood constituents of rats and humans

J. M. Wirsing, L. W.D. Weber, A. A. Kettrup, Karl K. Rozman

Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224098
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

Whole blood or plasma from male human volunteers or pooled from male Sprague-Dawley rats was incubated with varying amounts of ³H-2,3,7,8-tetrachlorodibenzo-p-dioxin (³H-TCDD). Blood was separated into cellular, protein and lipoprotein fractions by centrifugation. The distribution of ³H-TCDD between lipoproteins and plasma proteins was independent of ³H-TCDD concentration in the range of 65 fmol-1 nmol/ml plasma. The distribution of ³H-TCDD between the various lipoprotein fractions depended only on their relative content of total cholesterol plus triglycerides. The partitioning of ³H- TCDD between lipoproteins and plasma proteins was inversely proportional, whereas the distribution between the cellular fraction and the lipoproteins was directly proportional to the total plasma cholesterol plus triglyceride content. As a consequence of species differences in blood composition, the major part of ³H-TCDD-associated radioactivity was recovered from lipoproteins in human blood but from erythrocytes in rat blood. A mathematical description of the distribution of TCDD between blood components is presented.

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J. M. Wirsing, L. W.D. Weber, A. A. Kettrup, and Karl K. Rozman "Distribution of TCDD in blood constituents of rats and humans", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224098

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KEYWORDS

Plasma

Blood

Proteins

Information operations

Tissues

Silicon

Magnesium

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