Laser diffractometer of RBC suspension

Igor Z. Nemtsev

doi:10.1117/12.239539

10 May 1996 Laser diffractometer of RBC suspension

Igor Z. Nemtsev

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239539
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The original optical diagnostic device for measuring the RBC membrane permeability and RBC charge is considered in this message. A blood microsample drips in the mixer filled with a solution of NaCl. The resultant RBC suspension trickles down the pipe into the drain vessel. The flat thin cell is fitted into the pipe. The optical channel consists of He-Ne laser whose beam goes through the flat cell perpendicularly to its sides and scatters by the RBC flowing through the thin cell. The scattered light falls on a frosted screen put in the focal plane of a lens. As the RBC concentration is more than 0.1% of suspension volume, RBC form two flows moving along slightly heparinized sides of the thin cell due to repel each other electrostatically. The two flows orient each other so that RBC round bases are perpendicular to the sides of the flat cell due to RBC dipole momentum. In this case RBC viewed from the side will form on the screen a visible diffraction ellipse with axes lengths related in the initial time as 4:1. The measurement of the rate of the changes of the lengths of the axes of the diffraction ellipse due to osmos made it possible to develop a number of original optical diagnostic techniques approved by clinical practice. The method of measuring the membrane permeability was approbated clinically by examining blood samples (50 mcl) of 30 patients suffering from heavy poisoning by alcohol and barbiturates before and after detoxifying treatment and allowed the use the method developed for diagnosis the degree of poisoning and choosing the appropriate detoxifying rehabilitation. Unlike the ectacytometer where the shear stress between two planes is constant, the device offered has an area in the center of the cell with zero shear stress. It is the area where RBC should go with the increasing shear stress in the cell. The electric charge of RBC prevent them from going to the central plane, loosing mutual orientation, and can be measured.

Citation Download Citation

Igor Z. Nemtsev "Laser diffractometer of RBC suspension", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239539

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KEYWORDS

Diffraction

Blood

Channel projecting optics

Helium neon lasers

Light scattering

Optical diagnostics

Diagnostics

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