Paper
26 April 1996 Control of the activity of an enzyme immobilized into thermoreversible hydrogel
Larisa V. Sigolaeva, Nicolay L. Eremeev, Novella F. Kazanskaya
Author Affiliations +
Abstract
The effect of phase transition of a thermosensitive matrix on temperature dependencies of observed activity, equilibrium, and kinetic constants of the enzymatic reaction was studied using (alpha) -chymotrypsin immobilized into thermosensitive poly-N-isopropylacrylamide gel as a model system. The behavior of the system is determined by the properties of the enzyme at temperatures less than critical temperature corresponding to phase transition of the polymeric gel but by the properties of the thermosensitive polymeric matrix at temperatures higher than that critical temperature. The phase transition of the thermosensitive gel was shown to affect weakly temperature dependencies of equilibrium parameters of enzymatic reactions. All reversible effects of the reduction of the enzymatic activity at high temperature are exclusively due to the changes in temperature behavior of kinetic parameters of the enzymatic reaction.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Larisa V. Sigolaeva, Nicolay L. Eremeev, and Novella F. Kazanskaya "Control of the activity of an enzyme immobilized into thermoreversible hydrogel", Proc. SPIE 2779, 3rd International Conference on Intelligent Materials and 3rd European Conference on Smart Structures and Materials, (26 April 1996); https://doi.org/10.1117/12.237023
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Polymers

Proteins

Diffusion

Catalysis

Systems modeling

Ionization

Knowledge management

RELATED CONTENT

Single-molecule microscopy using tunable nanoscale confinement
Proceedings of SPIE (September 11 2013)
Cell as a factory for humanized encapsulation
Proceedings of SPIE (February 23 2012)
Dynamic light-scattering studies of mucin
Proceedings of SPIE (July 21 1993)
Probe diffusion in polymer solutions
Proceedings of SPIE (June 01 1991)
Kinetics of chemically amplified resists
Proceedings of SPIE (June 09 1995)

Back to Top