Novel optoanalytical method for the determination of pesticide residues

Ashutosh Sharma; Nigel S. M. Quantrill; Kim R. Rogers

doi:10.1117/12.260999

10 December 1996 Novel optoanalytical method for the determination of pesticide residues

Ashutosh Sharma, Nigel S. M. Quantrill, Kim R. Rogers

Proceedings Volume 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII; (1996) https://doi.org/10.1117/12.260999
Event: SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, 1996, Denver, CO, United States

Abstract

A new method for the measurement of pesticide residues is described that utilizes an excited state photo-reaction in conjunction with a dehydrogenase enzyme based enzymatic reaction. The presence of the pesticide inhibits the enzymatic reaction, thereby affecting the production of the NADH. The latter co-factor undergoes an excited state photo- reduction, resulting in the NAD⁺ and photo-reduced form of the indicator fluorophore. The reported method is suitable for analyzing a number of pesticides, selectively. Various advantages, including the possibilities of using the assay in complex matrices, non-separation formats and cost- effectiveness, are discussed.

Citation Download Citation

Ashutosh Sharma, Nigel S. M. Quantrill, and Kim R. Rogers "Novel optoanalytical method for the determination of pesticide residues", Proc. SPIE 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII, (10 December 1996); https://doi.org/10.1117/12.260999

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