Tumor detection in HpD-sensitized mice with fluorescence lifetime imaging

Rinaldo Cubeddu; Gianfranco L. Canti; Antonio Pifferi; Paola Taroni; Gianluca Valentini

doi:10.1117/12.273499

8 May 1997 Tumor detection in HpD-sensitized mice with fluorescence lifetime imaging

Rinaldo Cubeddu, Gianfranco L. Canti, Antonio Pifferi, Paola Taroni, Gianluca Valentini

Proceedings Volume 2972, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VI; (1997) https://doi.org/10.1117/12.273499
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Three time-delayed fluorescence images were used to calculate the spatial distribution of the fluorescence decay time in tumor-bearing mice sensitized with Hematoporphyrin Derivative (HpD). This allowed us to observe that the drug has a significantly longer fluorescence lifetime when it is incorporated in neoplastic tissues with respect to healthy surrounding ones. The imaging technique can be applied noninvasively in vivo in real time. Its diagnostic potential was tested on two murine tumor models after sensitization with low doses of HpD. In all the experimental conditions considered, the tumor could be easily identified in the lifetime images. Images were acquired also after removal of the skin overlying the tumor mass. Removal of the skin led to higher values of the lifetime contrast and allowed an even easier tumor localization.

Citation Download Citation

Rinaldo Cubeddu, Gianfranco L. Canti, Antonio Pifferi, Paola Taroni, and Gianluca Valentini "Tumor detection in HpD-sensitized mice with fluorescence lifetime imaging", Proc. SPIE 2972, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VI, (8 May 1997); https://doi.org/10.1117/12.273499

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available