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23 May 1997 Laser microbeam abalation of GFP-labeled nuclear organelles in a living cell.
Vickie J. LaMorte, Tatiana B. Krasieva, Ronald M. Evans, Michael W. Berns, Bruce J. Tromberg
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Proceedings Volume 2983, Functional Imaging and Optical Manipulation of Living Cells; (1997) https://doi.org/10.1117/12.274333
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
Cancer, development, cellular growth and differentiation are governed by gene expression. Recent molecular and cellular advances to visualize and perturb the pathways of transcriptional regulation, nascent RNA processing, and protein trafficking at the single cell level have been developed. More recently, applications utilizing the green fluorescent marker (GFP) from Aequorea victoria have facilitated visualization of these molecular events in a living cell. Specifically, we will describe a novel approach to perturb cellular processes by labeling discrete cellular components of interest with GFP and subsequently altering/ablating them with a laser microbeam.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Vickie J. LaMorte, Tatiana B. Krasieva, Ronald M. Evans, Michael W. Berns, and Bruce J. Tromberg "Laser microbeam abalation of GFP-labeled nuclear organelles in a living cell.", Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); https://doi.org/10.1117/12.274333
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KEYWORDS
Laser ablation
Green fluorescent protein
Proteins
Luminescence
Visualization
Pulsed laser operation
Cancer
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