Autofluorescence of human epidermis under UV irradiation in vitro

Svetlana P. Chernova; Alexander B. Pravdin; E. V. Bukatova

doi:10.1117/12.266244

11 February 1997 Autofluorescence of human epidermis under UV irradiation in vitro

Svetlana P. Chernova, Alexander B. Pravdin, E. V. Bukatova

Proceedings Volume 3053, Nonlinear Dynamics and Structures in Biology and Medicine: Optical and Laser Technologies: International Workshop; (1997) https://doi.org/10.1117/12.266244
Event: International Workshop on Nonlinear Dynamics and Structures in Biology and Medicine: Optical and Laser Technologies, 1996, Saratov, Russian Federation

Abstract

We studied autofluorescence of the layers of normal human epidermis in the course of the development of less invasive technique for assessment of tissue state. Surface epidermal stripping technique was applied to spectrofluorimetric study of epidermis. Three bands (280 plus or minus 20 nm, 360 plus or minus 20 nm, 435 plus or minus 20 nm) were used for excitation of sample fluorescence. Some well reproducible autofluorescence bands were observed over all samples. Data averaged over the group of volunteers are indicative of the decrease of intrinsic epidermis fluorophores content with depth. Alterations in fluorescence of samples of different epidermal layers under storage were studied as well as the dynamics of autofluorescence degradation in just taken strippings under continuous UV irradiation.

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Svetlana P. Chernova, Alexander B. Pravdin, and E. V. Bukatova "Autofluorescence of human epidermis under UV irradiation in vitro", Proc. SPIE 3053, Nonlinear Dynamics and Structures in Biology and Medicine: Optical and Laser Technologies: International Workshop, (11 February 1997); https://doi.org/10.1117/12.266244

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