Porphyrin binding with blood cells

Vladimir P. Zorin; Ivan I. Khludeyev; Valery P. Savitsky; Sergey B. Mel'nov; Nina D. Kochubeyeva

doi:10.1117/12.297824

29 December 1997 Porphyrin binding with blood cells

Vladimir P. Zorin, Ivan I. Khludeyev, Valery P. Savitsky, Sergey B. Mel'nov, Nina D. Kochubeyeva

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297824
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Using fluorescence activated cell sorting we have compared the binding of a number of porphyrins with different polarity by blood cells. According to pigment level blood cells may be arranged in order granulocytes greater than or equal to monocytes greater than lymphocytes greater than erythrocytes. Cellular accumulation of selected porphyrins in blood cells was remarkably different. The equilibrium level of chlorin e6 dimethylester in blood cells was about 15 times higher compared with chlorin e6. As a result, the percentage of pigment binding by blood cells varied from 0% (of total amount) in the case of polar pigments to about 50% for moderately apolar porphyrins. The results obtained show that pigment binding to blood cells may be of certain value when the pharmacokinetic behavior of porphyrin sensitizer is analyzed.

Citation Download Citation

Vladimir P. Zorin, Ivan I. Khludeyev, Valery P. Savitsky, Sergey B. Mel'nov, and Nina D. Kochubeyeva "Porphyrin binding with blood cells", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297824

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