Mapping the distribution of zinc phthalocyanine derivatives in EAhy 926 cells using Raman microscopy

Tami L. Freeman; Sarah E. Cope; Mark R. Stringer; Janet E. Cruse-Sawyer; Stanley B. Brown; David N. Batchelder

doi:10.1117/12.297981

29 December 1997 Mapping the distribution of zinc phthalocyanine derivatives in EAhy 926 cells using Raman microscopy

Tami L. Freeman, Sarah E. Cope, Mark R. Stringer, Janet E. Cruse-Sawyer, Stanley B. Brown, David N. Batchelder

Author Affiliations +

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297981
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

We have applied Raman spectroscopy to the study of the distribution within cells of photosensitizing compounds designed for use in photodynamic therapy. A human endothelial hybridoma cell-line was incubated in the presence of substituted zinc phthalocyanines, with the cells subsequently fixed in formalin. Microscopic Raman analysis was performed using 782 nm excitation in order to avoid photosensitizer absorption, thereby minimizing both the background fluorescence signal and the generation of cytotoxic species. A method of Raman mapping is described and we demonstrate that it is possible to identify the distribution of photosensitizer within the cell. The results indicate that sub-cellular photosensitizer localization is dependent upon both molecular structure and incubation time.

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Tami L. Freeman, Sarah E. Cope, Mark R. Stringer, Janet E. Cruse-Sawyer, Stanley B. Brown, and David N. Batchelder "Mapping the distribution of zinc phthalocyanine derivatives in EAhy 926 cells using Raman microscopy", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297981

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