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Ischemia-reperfusion injury is the leading cause of early dysfunction following transplantation. Currently, there are no techniques available to accurately measure ischemic changes during organ storage. Therefore, the interest exists in developing non-invasive monitoring techniques. We used NADH and tryptophan as fluorescent markers, since both are intrinsic fluorophores and excellent indicators for levels of hypoxia and protein denaturation, respectively.
Adrian Hamburger,Zygmunt Gryczynski,Joseph R. Lakowicz, andKeith Sommers M.D.
"Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium", Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351040
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Adrian Hamburger, Zygmunt Gryczynski, Joseph R. Lakowicz, Keith Sommers M.D., "Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium," Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); https://doi.org/10.1117/12.351040