Surface-enhanced Raman spectroscopy of bacteria coated by silver

Schlomo Efrima; Burt V. Bronk; Jozsef Czege

doi:10.1117/12.347519

3 May 1999 Surface-enhanced Raman spectroscopy of bacteria coated by silver

Schlomo Efrima, Burt V. Bronk, Jozsef Czege

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347519
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We present a novel method to measure Raman spectra from whole bacteria cells by using Surface Enhanced Raman Scattering (SERS). We deposit a silver coat on Escherichia coli and Bacillus megaterium bacteria and measure strongly enhanced (greater than 400,000 fold) and highly reproducible Raman spectra. The spectra are rich but not overly congested, as the surface enhancement is selective to the precise chemical nature of the biochemical molecules, and their proximity to the silver particulate matter. The main bands we observe can be associated with peptides and polysaccharides in the cell- wall and its membrane. The spectra from E. coli (a Gram- negative bacterium) and B. megaterium (a Gram-positive bacterium) are similar in their general form, but differ in detail. The spectrum from a commercial yeast extract is vastly different. This approach can be extended to probe the internal chemical environment within bacteria and applied to the identification of micro-organisms also applied to studying other biochemical problems and phenomena, such as biomineralization, heavy metal toxicity, cell-wall structure and others.

Citation Download Citation

Schlomo Efrima, Burt V. Bronk, and Jozsef Czege "Surface-enhanced Raman spectroscopy of bacteria coated by silver", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347519

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