Third-harmonic generation (THG) imaging: 3D contrast without absorption

Michiel Muller; Jeffrey A. Squier; G. J. Brakenhoff

doi:10.1117/12.347556

6 May 1999 Third-harmonic generation (THG) imaging: 3D contrast without absorption

Michiel Muller, Jeffrey A. Squier, G. J. Brakenhoff

Proceedings Volume 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI; (1999) https://doi.org/10.1117/12.347556
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Recently a novel imaging technique based on third-harmonic generation (THG) was introduced. This technique relies on a third-order non-linear interaction to generate a coherent signal response on the third-harmonic frequency with respect to the fundamental input radiation. Here we report on the input NA dependence of the THG signal and examine the resulting imaging characteristics of this novel technique in terms of resolution and contrast generation. We'll demonstrate the potential of the technique through a number of imaging examples, with special emphasis on in vivo applications. The latter illustrates the non-invasive character of the technique.

Citation Download Citation

Michiel Muller, Jeffrey A. Squier, and G. J. Brakenhoff "Third-harmonic generation (THG) imaging: 3D contrast without absorption", Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); https://doi.org/10.1117/12.347556

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