Real-time confocal two-photon fluorescence microscope using a rotating microlens array

Katsumasa Fujita; Tomoyuki Kaneko; Osamu Nakamura; Masahito Oyamada; Tetsuro Takamatsu; Satoshi Kawata

doi:10.1117/12.347844

7 May 1999 Real-time confocal two-photon fluorescence microscope using a rotating microlens array

Katsumasa Fujita, Tomoyuki Kaneko, Osamu Nakamura, Masahito Oyamada, Tetsuro Takamatsu, Satoshi Kawata

Author Affiliations +

Proceedings Volume 3740, Optical Engineering for Sensing and Nanotechnology (ICOSN '99); (1999) https://doi.org/10.1117/12.347844
Event: Optical Engineering for Sensing and Nanotechnology (ICOSN '99), 1999, Yokohama, Japan

Abstract

The use of a confocal pinhole-array in a real-time two-photon fluorescence microscope with a microlens array is proposed to increase the three-dimensional resolution and the depth- penetration property. Enhancement of the lateral and axial resolution in the proposed microscope is observed in our experiments. Comparisons of images taken with and without the pinhole-array to verify the sharp depth-imaging performance are shown.

Citation Download Citation

Katsumasa Fujita, Tomoyuki Kaneko, Osamu Nakamura, Masahito Oyamada, Tetsuro Takamatsu, and Satoshi Kawata "Real-time confocal two-photon fluorescence microscope using a rotating microlens array", Proc. SPIE 3740, Optical Engineering for Sensing and Nanotechnology (ICOSN '99), (7 May 1999); https://doi.org/10.1117/12.347844

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