Characterization of type I, II, III, IV, and V collagens by time-resolved laser-induced fluorescence spectroscopy

Laura Marcu; David Cohen; Jean-Michel I. Maarek; Warren S. Grundfest M.D.

doi:10.1117/12.382720

19 April 2000 Characterization of type I, II, III, IV, and V collagens by time-resolved laser-induced fluorescence spectroscopy

Laura Marcu, David Cohen, Jean-Michel I. Maarek, Warren S. Grundfest M.D.

Author Affiliations +

Proceedings Volume 3917, Optical Biopsy III; (2000) https://doi.org/10.1117/12.382720
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The relative proportions of genetically distinct collagen types in connective tissues vary with tissue type and change during disease progression, development, wound healing, aging. This study aims to 1) characterize the spectro- temporal fluorescence emission of fiber different types of collagen and 2) assess the ability of time-resolved laser- induced fluorescence spectroscopy to distinguish between collagen types. Fluorescence emission of commercially available purified samples was induced with nitrogen laser excitation pulses and detected with a MCP-PMT connected to a digital storage oscilloscope. The recorded time-resolved emission spectra displayed distinct fluorescence emission characteristics for each collagen type. The time domain information complemented the spectral domain intensity data for improved discrimination between different collagen types. Our results reveal that analysis of the fluorescence emission can be used to characterize different species of collagen. Also, the results suggest that time-resolved spectroscopy can be used for monitoring of connective tissue matrix composition changes due to various pathological and non-pathological conditions.

Citation Download Citation

Laura Marcu, David Cohen, Jean-Michel I. Maarek, and Warren S. Grundfest M.D. "Characterization of type I, II, III, IV, and V collagens by time-resolved laser-induced fluorescence spectroscopy", Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); https://doi.org/10.1117/12.382720

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