Laser phase microscopy and functional imaging of living human cancer cells during the cell cycle

Elena V. Perevedentseva; Georgi Graschew; Evangelos Balanos; Cathrin Dressler; Juergen Beuthan; Peter M. Schlag

doi:10.1117/12.384183

2 May 2000 Laser phase microscopy and functional imaging of living human cancer cells during the cell cycle

Elena V. Perevedentseva, Georgi Graschew, Evangelos Balanos, Cathrin Dressler, Juergen Beuthan, Peter M. Schlag

Proceedings Volume 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII; (2000) https://doi.org/10.1117/12.384183
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The purpose of the investigation was to elaborate a new method of functional imaging of living tumor cells. Human colon carcinoma cells HCT116 were investigated with a conventional light microscope, confocal laser scanning microscope and with a laser phase microscope (LPM). The LPM is a functional imaging technique providing information about cell morphology which is imposed by the physiological inhomogeneity of the refractive index. The phase of the light wave passing through an object contains quantitative information about the object thickness, the shape, and the spatial distribution of the refractive index varying with morphology and chemical composition inhomogeneity inside the object. The new method of investigation of the cells in different stages of the cell cycle is developed. Every phase image of the investigated cells has been compared with conventional light microscopic and confocal microscopic images of the same cell. the relation between the cell state, their morphological peculiarities and the phase characteristics of the measured cell is determined. Data thus acquired, quantitatively characterizing intra- and intercellular processes during the cell cycle, and the method of measurements can be used to investigate with high optic resolution the mechanisms of different physical, chemical and biomolecular interactions with the tumor cells.

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Elena V. Perevedentseva, Georgi Graschew, Evangelos Balanos, Cathrin Dressler, Juergen Beuthan, and Peter M. Schlag "Laser phase microscopy and functional imaging of living human cancer cells during the cell cycle", Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); https://doi.org/10.1117/12.384183

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KEYWORDS

Phase shifts

Microscopes

Functional imaging

Confocal microscopy

Refractive index

Microscopy

Phase measurement

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