Imaging through turbulence with the volume-holographic confocal microscope

Michal Balberg; George Barbastathis; David J. Brady

doi:10.1117/12.406352

15 December 2000 Imaging through turbulence with the volume-holographic confocal microscope

Michal Balberg, George Barbastathis, David J. Brady

Proceedings Volume 4087, Applications of Photonic Technology 4; (2000) https://doi.org/10.1117/12.406352
Event: 2000 International Conference on Application of Photonic Technology (ICAPT 2000), 2000, Quebec City, Canada

Abstract

The confocal microscope with volume-holographic collector [1] utilizes Bragg selectivity in order to achieve depth sectioning. The volume hologram is recorded by the interference of two beams, one of which originates as a point source at a reference depth. When a reconstructing Qrobe) source is at the reference depth, it is Bragg-matched and causes a strong diffracted signal; sources at different depths are rejected because of Bragg mismatch. Therefore, the use of a pinhole in front of the detector (as in traditional confocal microscopes [2]) is not required. The arguments for use of a volume hologram instead of a pinhole to achieve depth sectioning are: (i) the depth resolution of the microscope is independent of its photon-collection performance; (ii) the hologram phase conjugates aberrations and other systematic phase distortions, and hence acts as an ideal matched filter to the reference source (unlike the ad hoc filtering function performed by the pinhole). In this paper we focus on the depth-resolving properties of volume diffraction when a turbulent (scattering) medium, emulating a biological tissue, is present in the path of the light entering the microscope. In particular, we compare the depth resolution of a microscope recorded with and without precompensation for the presence of the scatterer. Precompensation extends the matched filtering principle by use of a priori information (the thickness of the turbulent medium) during the recording phase of the hologram. Our experimental results (section 2) demonstrate that precompensation increases the scatterer thickness over which confocal imaging is possible.

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Michal Balberg, George Barbastathis, and David J. Brady "Imaging through turbulence with the volume-holographic confocal microscope", Proc. SPIE 4087, Applications of Photonic Technology 4, (15 December 2000); https://doi.org/10.1117/12.406352

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KEYWORDS

Microscopes

Holograms

Confocal microscopy

Scattering

Polymers

Silicon

Turbulence

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