Morphological slice-based counting of illuminated 3D bodies

Artyom M. Grigoryan; Edward R. Dougherty

doi:10.1117/12.424988

8 May 2001 Morphological slice-based counting of illuminated 3D bodies

Artyom M. Grigoryan, Edward R. Dougherty

Proceedings Volume 4304, Nonlinear Image Processing and Pattern Analysis XII; (2001) https://doi.org/10.1117/12.424988
Event: Photonics West 2001 - Electronic Imaging, 2001, San Jose, CA, United States

Abstract

This paper presents a simulation toolbox for counting illuminated 3D bodies. The current model is limited to illuminated balls but can be extended to other illuminated bodies. Upon simulation of a set of balls in space, horizontal slices are taken to provide a stack of 2D gray- scale images. Based on these images, a morphological algorithm estimates the number of balls. Construction of the toolbox has been motivated by the need to count spots in FISH images to test for elevated gene copy numbers. The toolbox facilitates analysis of various algorithm parameters based on the distribution of the 3D bodies. These include the number of slices and various settings for the morphological filters composing the algorithm.

Citation Download Citation

Artyom M. Grigoryan and Edward R. Dougherty "Morphological slice-based counting of illuminated 3D bodies", Proc. SPIE 4304, Nonlinear Image Processing and Pattern Analysis XII, (8 May 2001); https://doi.org/10.1117/12.424988

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