Kinetics of photo processes in dye molecules and the resolution ofa two-photon excitation fluorescence microscope with nanosecond laser

Evgeny Kleymionov; Valery S. Ivanov; Niko Meltola; Pekka E. Hanninen; Erkki J. Soini

doi:10.1117/12.407664

27 November 2000 Kinetics of photo processes in dye molecules and the resolution ofa two-photon excitation fluorescence microscope with nanosecond laser

Evgeny Kleymionov, Valery S. Ivanov, Niko Meltola, Pekka E. Hanninen, Erkki J. Soini

Author Affiliations +

Proceedings Volume 4316, International Conference on Lasers for Measurements and Information Transfer; (2000) https://doi.org/10.1117/12.407664
Event: International Conference on Lasers for Measurements and Information Transfer, 2000, St. Petersburg, Russian Federation

Abstract

The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.

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Evgeny Kleymionov, Valery S. Ivanov, Niko Meltola, Pekka E. Hanninen, and Erkki J. Soini "Kinetics of photo processes in dye molecules and the resolution ofa two-photon excitation fluorescence microscope with nanosecond laser", Proc. SPIE 4316, International Conference on Lasers for Measurements and Information Transfer, (27 November 2000); https://doi.org/10.1117/12.407664

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