Controlling actin motility on microfabricated linear channels

Chitladda Mahanivong; Jonathan P. Wright; Murat Kekic; Duy K. Pham; Cristobal dos Remedios; Dan V. Nicolau

doi:10.1117/12.454621

19 November 2001 Controlling actin motility on microfabricated linear channels

Chitladda Mahanivong, Jonathan P. Wright, Murat Kekic, Duy K. Pham, Cristobal dos Remedios, Dan V. Nicolau

Proceedings Volume 4590, BioMEMS and Smart Nanostructures; (2001) https://doi.org/10.1117/12.454621
Event: International Symposium on Microelectronics and MEMS, 2001, Adelaide, Australia

Abstract

Heavy meromyosin (HMM), a proteolytically cleaved derivative of myosin has previously been shown to interact with actin in well-established in vitro motility assays on nitrocellulose surfaces. In this study, the assays were conducted to demonstrate that the motility of actin filaments is confined in the micron-sized channels fabricated via laser ablation in a layer of the photosensitive resist polymer (O-acryloyloxime acetophenone oxime, AAPO). A solution containing myosin labelled with fluorophore 5-iodoacetamidofluorescein (5-IAF) was applied to the microfabricated AAPO surface and shown to bind specifically to the micron-size channels. In the motility assay, HMM, rhodamine-phalloidin labelled actin and ATP were sequentially added and the movement of the actin filaments was observed by fluorescence microscopy and recorded with a CCD camera. The experiments prove that although the actin filaments show an only-partial propensity for attachment in myosin-rich areas, their motility is confined to a large extent in micro- channels.

Citation Download Citation

Chitladda Mahanivong, Jonathan P. Wright, Murat Kekic, Duy K. Pham, Cristobal dos Remedios, and Dan V. Nicolau "Controlling actin motility on microfabricated linear channels", Proc. SPIE 4590, BioMEMS and Smart Nanostructures, (19 November 2001); https://doi.org/10.1117/12.454621

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