Hydrogen peroxide production in cultured RPE cells exposed to near-infrared lasers

Michael L. Denton; Debbie M. Eikum; David J. Stolarski; Gary D. Noojin; Robert J. Thomas; Randolph D. Glickman; Benjamin A. Rockwell

doi:10.1117/12.472518

27 June 2002 Hydrogen peroxide production in cultured RPE cells exposed to near-infrared lasers

Michael L. Denton, Debbie M. Eikum, David J. Stolarski, Gary D. Noojin, Robert J. Thomas, Randolph D. Glickman, Benjamin A. Rockwell

Author Affiliations +

Proceedings Volume 4617, Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical; (2002) https://doi.org/10.1117/12.472518
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Hydrogen peroxide (H₂O₂) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H₂O₂ as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode- locked laser exposure.

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Michael L. Denton, Debbie M. Eikum, David J. Stolarski, Gary D. Noojin, Robert J. Thomas, Randolph D. Glickman, and Benjamin A. Rockwell "Hydrogen peroxide production in cultured RPE cells exposed to near-infrared lasers", Proc. SPIE 4617, Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical, (27 June 2002); https://doi.org/10.1117/12.472518

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