Cell-by-cell construction of living tissue

Bradley R. Ringeisen; Heungsoo Kim; Henry D. Young; Barry J. Spargo; Raymond C. Y. Auyeung; Alberto Pique; Douglas B. Chrisey; Peter K. Wu

doi:10.1117/12.470640

18 June 2002 Cell-by-cell construction of living tissue

Bradley R. Ringeisen, Heungsoo Kim, Henry D. Young, Barry J. Spargo, Raymond C. Y. Auyeung, Alberto Pique, Douglas B. Chrisey, Peter K. Wu

Author Affiliations +

Proceedings Volume 4637, Photon Processing in Microelectronics and Photonics; (2002) https://doi.org/10.1117/12.470640
Event: High-Power Lasers and Applications, 2002, San Jose, California, United States

Abstract

This paper outlines investigations into a potentially revolutionary approach to tissue engineering. Tissue is a complex 3D structure that contains many different biomaterials such as cells, proteins, and extracellular matrix molecules that are ordered in a very precise way to serve specific functions. In order to replicate such complex structure, it is necessary to have a tool that could deposit all these materials in an accurate and controlled fashion. Most methods to fabricate living 3D structures involve techniques to engineer biocompatible scaffolding, which is then seeded with living cells to form tissue. This scaffolding gives the tissue needed support, but the resulting tissue inherently has no microscopic cellular structure because cells are injected into the scaffolding where they adhere ta random. Wee have developed a novel technique that actually engineers tissue, not scaffolding, that includes the mesoscopic cellular structure inherent in natural tissues. This approach uses a laser-based rapid prototyping system known as matrix assisted pulsed laser evaporation direct write to construct living tissue cell-by- cell. This manuscript details our efforts to rapidly and reproducibly fabricate comlpex 2D and 3D tissue structures with MAPLE-DW by placing different cells and biomaterials accurately and adherently on the mesoscopic scale.

Citation Download Citation

Bradley R. Ringeisen, Heungsoo Kim, Henry D. Young, Barry J. Spargo, Raymond C. Y. Auyeung, Alberto Pique, Douglas B. Chrisey, and Peter K. Wu "Cell-by-cell construction of living tissue", Proc. SPIE 4637, Photon Processing in Microelectronics and Photonics, (18 June 2002); https://doi.org/10.1117/12.470640

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available