Paper
27 November 2002 Nonlinear phase contrast using a bacteriorhodopsin film
Author Affiliations +
Abstract
In this paper we demonstrate a novel phase contrast system that employs a BR film. Since the filter is optically induced by the Fourier transform of the phase object, no alignment is necessary at the filter plane making it extremely robust. Due to the optical properties of BR films the phase filter can be induced with low light intensity levels. The material response allows operation at video frame rates, processing of high spatial resolution objects, and the use of relatively inexpensive laser sources. Such characteristics and the fact that BR films can be produced at a low cost makes the system simple to implement, relatively inexpensive and extremely robust. The effects of varying the illuminating area beyond the phase object area and filter saturation are also analyzed.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Marcelo David Iturbe Castillo, J. David Sanchez-de-la-Llave, Ruben Ramos Garcia, Eduardo Tepichin-Rodriguez, and L. I. Olivos-Perez "Nonlinear phase contrast using a bacteriorhodopsin film", Proc. SPIE 4789, Algorithms and Systems for Optical Information Processing VI, (27 November 2002); https://doi.org/10.1117/12.456519
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KEYWORDS
Phase contrast

Optical filters

Nonlinear filtering

Spherical lenses

Visibility

Fourier transforms

Image analysis

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