High-throughput screening system for the study of phototoxicity of photosensitizers in vitro

Irina G. Meerovich; Victoria V. Jerdeva; Gennadii A. Meerovich; Valentina M. Derkacheva; Alexander P. Savitsky

doi:10.1117/12.480277

13 June 2003 High-throughput screening system for the study of phototoxicity of photosensitizers in vitro

Irina G. Meerovich, Victoria V. Jerdeva, Gennadii A. Meerovich, Valentina M. Derkacheva, Alexander P. Savitsky

Proceedings Volume 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII; (2003) https://doi.org/10.1117/12.480277
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

The technique and the device for studying of phototoxic properties of photosensitizers in vitro on cell monolayers in 96-well microplate was developed. It allows to irradiate independently each well of microtiter plate, and study simultaneously all points of dependence of phototoxic effect on the light dose for certain conditions of investigation. Also it allows to study and compare at the same conditions the dependence of phototoxic effect on the light dose for different concentrations of photosensitizer or for several photosensitizers. Developed device includes powerful source of light based arc xenon lamp with elliptical reflector, special filters and multi-fiber bundle. To determine the degree of the cell viability under the photodynamic treatment proliferative test with fluoresceinediacetate was used. Using this device and technique phototoxic action of a number of different derivatives of aluminium phthalocyanines was studied. The results of this screening have a good correlation with the results obtained in vivo on mice.

Citation Download Citation

Irina G. Meerovich, Victoria V. Jerdeva, Gennadii A. Meerovich, Valentina M. Derkacheva, and Alexander P. Savitsky "High-throughput screening system for the study of phototoxicity of photosensitizers in vitro", Proc. SPIE 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII, (13 June 2003); https://doi.org/10.1117/12.480277

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