New implementation of second harmonic generation microscopy for three-dimensional resolution

Changhuei Yang; Jerome Mertz

doi:10.1117/12.478032

10 July 2003 New implementation of second harmonic generation microscopy for three-dimensional resolution

Changhuei Yang, Jerome Mertz

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478032
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We present a fast scanning transmission-mode confocal scanning laser microscope system based on the use of a second harmonic generation (SHG) crystal for signal detection. The quadratic intensity dependence of SHG is exploited to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal plays the role of a virtual pinhole that remains self-aligned without a need for de-scanning. We demonstrate that this new microscope method produces images with higher contrast and less speckle than transmission scanning microscopy with linear detection.

Citation Download Citation

Changhuei Yang and Jerome Mertz "New implementation of second harmonic generation microscopy for three-dimensional resolution", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478032

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