Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses

Hiroyuki Kawano; Yasuo Nabekawa; Akira Suda; Yu Oishi; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

doi:10.1117/12.500445

14 October 2003 Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses

Hiroyuki Kawano, Yasuo Nabekawa, Akira Suda, Yu Oishi, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Author Affiliations +

Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500445
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Two-photon excited fluorescence process of green fluorescent protein was adaptively controlled by shaped femtosecond excitation laser pulses. The phase control was given by a fused-silica spatial light modulator. We could completely intentionally increase and decrease of fluorescence efficiency against the excitation intensity. This fully-controllable method can become a robust solution for various practical problems such as photobleaching of the samples on a two-photon fluorescence microscopy.

Citation Download Citation

Hiroyuki Kawano, Yasuo Nabekawa, Akira Suda, Yu Oishi, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa "Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500445

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