From research to routines: ultrafast laser systems can be replaced by low-cost lasers in two-photon excitation applications

Marko Eerik Tirri; Jonne Vaarno; Juhani T. Soini; Pekka E. Hanninen

doi:10.1117/12.500467

14 October 2003 From research to routines: ultrafast laser systems can be replaced by low-cost lasers in two-photon excitation applications

Marko Eerik Tirri, Jonne Vaarno, Juhani T. Soini, Pekka E. Hanninen

Author Affiliations +

Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500467
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

This paper outlines the latest developments in two-photon excitation laboratory instrumentation using low-cost laser equipment. Our research group has shown that application of two-photon excitation need not to be limited to those laboratories that can afford the price and maintenance of ultrafast laser systems. With certain compromises and well-designed experiments, low-cost lasers, such as the Q-switched Nd:YAG microchip laser, can be used in utilizing the positive properties of two-photon excitation. Results using microparticles or cells as bioactive carriers confirm this fact. Application examples from biomedical research as well from clinical environment are presented.

Citation Download Citation

Marko Eerik Tirri, Jonne Vaarno, Juhani T. Soini, and Pekka E. Hanninen "From research to routines: ultrafast laser systems can be replaced by low-cost lasers in two-photon excitation applications", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500467

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