Biocompound detection through fluorescence excitation-emission matrix analysis

David R. Twede; Lee C. Sanders; Michael L. Wagner

doi:10.1117/12.506169

7 January 2004 Biocompound detection through fluorescence excitation-emission matrix analysis

David R. Twede, Lee C. Sanders, Michael L. Wagner

Proceedings Volume 5159, Imaging Spectrometry IX; (2004) https://doi.org/10.1117/12.506169
Event: Optical Science and Technology, SPIE's 48th Annual Meeting, 2003, San Diego, California, United States

Abstract

The excitation-emission matrix (EEM) is the luminescence spectral emission intensity of fluorescent compounds as a function of the excitation wavelength. EEMs offer the promise of an additional degree of information for enhanced compound detection and identification. Veridian has collected pure-component EEMs of amino acids (Trp, Phe, Tyr), Bacillus globigii (bg), Bacillus thuringiensis (bt,), and selected backgrounds. Also collected were EEMs of mixtures of amino acids and of bg in solution with a few backgrounds. The EEMs of pure components and mixtures were analyzed for phenomenology and for potential methods of unmixing and identifying the constituents of EEMs having mixed components of a similar nature.

Citation Download Citation

David R. Twede, Lee C. Sanders, and Michael L. Wagner "Biocompound detection through fluorescence excitation-emission matrix analysis", Proc. SPIE 5159, Imaging Spectrometry IX, (7 January 2004); https://doi.org/10.1117/12.506169

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