Fluorescence lifetime imaging microscopy based on a synchroscan streak camera

Junle Qu; Ji Li; Hanben Niu

doi:10.1117/12.530987

13 July 2004 Fluorescence lifetime imaging microscopy based on a synchroscan streak camera

Junle Qu, Ji Li, Hanben Niu

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.530987
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

A new time-domain two-dimensional fluorescence lifetime detection method and a fluorescence lifetime imaging microscope that is based on a synchroscan streak camera are presented. This system can generate in parallel a complete two-dimensional fluorescence lifetime image without scanning the light. We demonstrate the acquisition of a fluorescence lifetime image of a semiconductor ET material sample at a temporal resolution of 2ps and spatial resolution of 4μm within 40ms.

Citation Download Citation

Junle Qu, Ji Li, and Hanben Niu "Fluorescence lifetime imaging microscopy based on a synchroscan streak camera", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.530987

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