Spectrally resolved fluorescence lifetime and FRET measurements

Christoph U. Biskup; Laimonas Kelbauskas; Thomas Zimmer; Sascha Dietrich; Klaus Benndorf; Wolfgang Becker; Axel Bergmann; Nikolaj Klocker

doi:10.1117/12.613006

30 March 2005 Spectrally resolved fluorescence lifetime and FRET measurements

Christoph U. Biskup, Laimonas Kelbauskas, Thomas Zimmer, Sascha Dietrich, Klaus Benndorf, Wolfgang Becker, Axel Bergmann, Nikolaj Klocker

Author Affiliations +

Proceedings Volume 5700, Multiphoton Microscopy in the Biomedical Sciences V; (2005) https://doi.org/10.1117/12.613006
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We present two different approaches that allow multi-wavelength fluorescence lifetime measurements in the time domain in conjunction with a laser scanning microscope and a pulsed excitation source. One technique is based on a streak camera system, the other technique is based on a time-correlated-single-photon-counting (TCSPC) approach. When applied to Forster resonance energy transfer (FRET) measurements, these setups are capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.

Citation Download Citation

Christoph U. Biskup, Laimonas Kelbauskas, Thomas Zimmer, Sascha Dietrich, Klaus Benndorf, Wolfgang Becker, Axel Bergmann, and Nikolaj Klocker "Spectrally resolved fluorescence lifetime and FRET measurements", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); https://doi.org/10.1117/12.613006

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available