Monitoring of collagen shrinkage by use of second harmonic generation microscopy

Sung-Jan Lin; Jau-Shiuh Chen; Wen Lo; Yen Sun; Wei-Liang Chen; Jung-Yi Chan; Hsin-Yuan Tan; Wei-Chou Lin; Chih-Jung Hsu; Tai-Horng Young; Shiou-Hwa Jee; Chen-Yuan Dong

doi:10.1117/12.647087

1 March 2006 Monitoring of collagen shrinkage by use of second harmonic generation microscopy

Sung-Jan Lin, Jau-Shiuh Chen, Wen Lo, Yen Sun, Wei-Liang Chen, Jung-Yi Chan, Hsin-Yuan Tan, Wei-Chou Lin, Chih-Jung Hsu, Tai-Horng Young, Shiou-Hwa Jee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 60840S (2006) https://doi.org/10.1117/12.647087
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Thermal treatment induced collagen shrinkage has a great number of applications in medical practice. Clinically, the there is lack of reliable non-invasive methods to quantify the shrinkage. Overt treatment by heat application can lead to devastating results. We investigate the serial changes of collagen shrinkage by thermal treatment of rat tail tendons. The change in length is correlated with the finding in second harmonic generation microscopy and histology. Rat tail tendon shortens progressively during initial thermal treatment. After a certain point in time, the length then remains almost constant despite further thermal treatment. The intensity of second harmonic generation signals also progressively decreases initially and then remains merely detectable upon further thermal treatment. It prompts us to develop a mathematic model to quantify the dependence of collagen shrinkage on changes of SHG intensity. Our results show that SHG intensity can be used to predict the degree of collagen shrinkage during thermal treatment for biomedical applications.

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Sung-Jan Lin, Jau-Shiuh Chen, Wen Lo, Yen Sun, Wei-Liang Chen, Jung-Yi Chan, Hsin-Yuan Tan, Wei-Chou Lin, Chih-Jung Hsu, Tai-Horng Young, Shiou-Hwa Jee, and Chen-Yuan Dong "Monitoring of collagen shrinkage by use of second harmonic generation microscopy", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840S (1 March 2006); https://doi.org/10.1117/12.647087

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KEYWORDS

Collagen

Second-harmonic generation

Microscopy

Harmonic generation

Tissues

Biomedical optics

Confocal microscopy

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