Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy

Eddy Kuwana; Gracie Vargas-Adams; Todd C. Pappas; Anton V. Liopo; Massoud Motamedi

doi:10.1117/12.723959

23 March 2007 Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy

Eddy Kuwana, Gracie Vargas-Adams, Todd C. Pappas, Anton V. Liopo, Massoud Motamedi

Proceedings Volume 6424, Photonic Therapeutics and Diagnostics III; 64242L (2007) https://doi.org/10.1117/12.723959
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this work, we demonstrate the use of Two-Photon Fluorescence Lifetime Imaging Microscopy (TP-FLIM) for intracellular calcium ([Ca²⁺]i) measurement with a calcium sensitive fluorescent dye in a neuronal cell model and brain tissue. Calcium perturbations were induced via chemical stimulation and our results signify the potential of TP-FLIM for quantitative ([Ca²⁺]i) measurement within the physiological range of intracellular [Ca²⁺].

Citation Download Citation

Eddy Kuwana, Gracie Vargas-Adams, Todd C. Pappas, Anton V. Liopo, and Massoud Motamedi "Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy", Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242L (23 March 2007); https://doi.org/10.1117/12.723959

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