Erythrocytes three-dimensional morphology by digital holographic interference microscopy

T. V. Tishko; V. P. Titar; D. N. Tishko

doi:10.1117/12.802297

29 April 2008 Erythrocytes three-dimensional morphology by digital holographic interference microscopy

T. V. Tishko, V. P. Titar, D. N. Tishko

Proceedings Volume 7006, Lasers for Measurements and Information Transfer 2007; 70060O (2008) https://doi.org/10.1117/12.802297
Event: Lasers for Measurements and Information Transfer 2007, 2007, St. Petersburg, Russian Federation

Abstract

We have developed the digital holographic interference microscope (DHIM) for real time three - dimensional (3-D) imaging and quantitative study of phase microscopic objects (MOs). The instrument integrates holographic interference microscope with digital processing of interferograms. The experimental procedure, allowing calculating microscopic objects thickness and 3-D imaging from the measured phase shift, is presented. The method has been applied to study 3-D morphology of red blood cells in norm, in pathology and under influence of physical and chemical factors in vivo. It has been found that morphologic changes of erythrocyte population had nonspecific character. Our investigations have proved that erythrocytes 3-D morphology reflects the state of a living organism and the level of its biological response on different external factors influence.

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T. V. Tishko, V. P. Titar, and D. N. Tishko "Erythrocytes three-dimensional morphology by digital holographic interference microscopy", Proc. SPIE 7006, Lasers for Measurements and Information Transfer 2007, 70060O (29 April 2008); https://doi.org/10.1117/12.802297

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KEYWORDS

Blood

Molybdenum

Digital holography

Holography

Organisms

3D image processing

Pathology

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