Imaging EGFR distribution using surface-enhanced Raman spectroscopy

L. Lucas; X. K. Chen; A. Smith; M. Korbelik; H. Zeng; P. W. K. Lee; K. C. Hewitt

doi:10.1117/12.808337

18 February 2009 Imaging EGFR distribution using surface-enhanced Raman spectroscopy

L. Lucas, X. K. Chen, A. Smith, M. Korbelik, H. Zeng, P. W. K. Lee, K. C. Hewitt

Proceedings Volume 7192, Plasmonics in Biology and Medicine VI; 719206 (2009) https://doi.org/10.1117/12.808337
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The purpose of this study is to explore the feasibility of using Surface Enhanced Raman Spectroscopy (SERS) to image the distribution of Epidermal Growth Factor Receptor (EGFR) in cells. To accomplish this task, 30 nm gold nanoparticles (AuNPs) tagged with antibodies to EGFR (10¹² per ml) are incubated with cells (10⁶ per ml) of the A431 human epidermoid carcinoma cell line and normal human bronchial epithelial (NHBE) cells. Using the 632.8 nm excitation line of a He-Ne laser, Raman spectroscopy measurements are performed using a point mapping scheme. SERS signals are observed with an overall enhancement of 4-7 orders of magnitude. Raman intensity maps of the 1480 and 1583 cm^-1 peaks correlate well with the expected distribution of AuNPs and EGFR. Normal cells show little to no enhancement. The results therefore present a simple yet effective means to image EGFR over-expression.

Citation Download Citation

L. Lucas, X. K. Chen, A. Smith, M. Korbelik, H. Zeng, P. W. K. Lee, and K. C. Hewitt "Imaging EGFR distribution using surface-enhanced Raman spectroscopy", Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 719206 (18 February 2009); https://doi.org/10.1117/12.808337

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