Harmonic holography for three-dimensional cellular imaging

Chia-Lung Hsieh; Rachel Grange; Ye Pu; Demetri Psaltis

doi:10.1117/12.820801

6 May 2009 Harmonic holography for three-dimensional cellular imaging

Chia-Lung Hsieh, Rachel Grange, Ye Pu, Demetri Psaltis

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Proceedings Volume 7329, Three-Dimensional Imaging, Visualization, and Display 2009; 73290H (2009) https://doi.org/10.1117/12.820801
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO₃ nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from BaTiO₃ nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H²) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H² microscope.

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Chia-Lung Hsieh, Rachel Grange, Ye Pu, and Demetri Psaltis "Harmonic holography for three-dimensional cellular imaging", Proc. SPIE 7329, Three-Dimensional Imaging, Visualization, and Display 2009, 73290H (6 May 2009); https://doi.org/10.1117/12.820801

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