Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging

Chia-Lung Hsieh; Rachel Grange; Ye Pu; Demetri Psaltis

doi:10.1117/12.831500

1 July 2009 Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging

Chia-Lung Hsieh, Rachel Grange, Ye Pu, Demetri Psaltis

Proceedings Volume 7367, Advanced Microscopy Techniques; 73670D (2009) https://doi.org/10.1117/12.831500
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO₃ nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and broad flexibility in the choice of excitation wavelength. In this paper, we report the use of nanoparticles for cell imaging. We first stabilized the BaTiO₃ nanoparticles in suspension and characterized the optical properties. We also demonstrated the functionalization of BaTiO₃ nanoparticles by conjugating IgG antibody on the surface of the nanoparticles. These functionalized nanocrystals are capable of specific labeling the antigens of interest.

Citation Download Citation

Chia-Lung Hsieh, Rachel Grange, Ye Pu, and Demetri Psaltis "Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670D (1 July 2009); https://doi.org/10.1117/12.831500

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