In vivo imaging of pancreatic endocrine islets

Martin Villiger; Joan Goulley; Christophe Pache; Michael Friedrich; Anne Grapin-Botton; Paolo Meda; Rainer Leitgeb; Theo Lasser

doi:10.1117/12.831864

13 July 2009 In vivo imaging of pancreatic endocrine islets

Martin Villiger, Joan Goulley, Christophe Pache, Michael Friedrich, Anne Grapin-Botton, Paolo Meda, Rainer Leitgeb, Theo Lasser

Author Affiliations +

Proceedings Volume 7372, Optical Coherence Tomography and Coherence Techniques IV; 73720E (2009) https://doi.org/10.1117/12.831864
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Extended focus optical coherence microscope (xfOCM) circumvents the compromise between lateral resolution and depth of field by us of a Bessel-like illumination beam. The high sensitivity and parallel depth profiling of Fourier domain optical coherence tomography are preserved, and combined with high isotropic resolution of 1.5 - 2 μm. To comply with the requirements for in vivo measurements, beam scanning had to be implemented. We then performed measurements, first of excised pancreas, validated by standard immunohistochemistry, to investigate the structures that can be observed. For a quantitative analysis, a semi-automatic islet detection algorithm evaluated the islet size, position, contrast and homogeneity. The influence of streptozotocin on the signature of the islets was investigated in a next step. Finally, xfOCM was applied to make measurements of the murine pancreas in situ and in vivo, visualizing pancreatic lobules, ducts, blood vessels and individual islets of Langerhans.

Citation Download Citation

Martin Villiger, Joan Goulley, Christophe Pache, Michael Friedrich, Anne Grapin-Botton, Paolo Meda, Rainer Leitgeb, and Theo Lasser "In vivo imaging of pancreatic endocrine islets", Proc. SPIE 7372, Optical Coherence Tomography and Coherence Techniques IV, 73720E (13 July 2009); https://doi.org/10.1117/12.831864

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