Phase reconstruction of living human embryonic kidney 293 cells based on two off-axis holograms

Wenjing Zhou; Yingjie Yu; Yanhong Duan; Anand Asundi

doi:10.1117/12.838970

24 August 2009 Phase reconstruction of living human embryonic kidney 293 cells based on two off-axis holograms

Wenjing Zhou, Yingjie Yu, Yanhong Duan, Anand Asundi

Proceedings Volume 7375, ICEM 2008: International Conference on Experimental Mechanics 2008; 737502 (2009) https://doi.org/10.1117/12.838970
Event: International Conference on Experimental Mechanics 2008 and Seventh Asian Conference on Experimental Mechanics, 2008, Nanjing, China

Abstract

In this paper, we validate experimentally the potential of off-axis digital micro-holography for 3D image reconstruction of a live Human Embryonic Kidney 293(HEK293) cell that is widely used as transfection and expression. A subtraction method of two off-axis holograms to reconstruct the phase of the live microscopic object is discussed. The presented subtraction method can remove some main noise, for example, the quadratic phase aberration introduced by microscope objective (MO), other phase aberration introduced by the liquid in tank and other interference noise introduced by the optical parts. Thus an improvement in the measurement precision of live cells in aqueous solution is observed. The potential of this method is demonstrated by providing the phase reconstruction results of a phase grating and a single HEK293 cell. The results showed good correspondence to the actual character of HEK293 cell prove the capability of digital micro-holography as a tool to monitor the dynamic transfection process of the living HEK 293 cells.

Citation Download Citation

Wenjing Zhou, Yingjie Yu, Yanhong Duan, and Anand Asundi "Phase reconstruction of living human embryonic kidney 293 cells based on two off-axis holograms", Proc. SPIE 7375, ICEM 2008: International Conference on Experimental Mechanics 2008, 737502 (24 August 2009); https://doi.org/10.1117/12.838970

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