Clearance of protoporphyrin IX induced by 5-aminolevulinic acid from WiDr human colon carcinoma cells

Asta Juzeniene; Miron Kaliszewski; Andrzej Bugaj; Johan Moan

doi:10.1117/12.822944

13 July 2009 Clearance of protoporphyrin IX induced by 5-aminolevulinic acid from WiDr human colon carcinoma cells

Asta Juzeniene, Miron Kaliszewski, Andrzej Bugaj, Johan Moan

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73802Q (2009) https://doi.org/10.1117/12.822944
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is the most widely practiced form of PDT in dermatology. One of the advantages of ALA-PDT is that undesirable photosensitization lasts only for 24-48 h. In order to optimize ALA-PDT it is necessary to understand the mechanisms controlling intracellular PpIX clearance (efflux and transformation into heme) in order to decrease protoporphyrin IX (PpIX) clearance rates in the early stages of its production. The aim of this study was to investigate the factors controlling the clearance of intracellular PpIX. Fluorescence spectroscopy was used to study PpIX kinetics in WiDr cells initially treated with ALA. The clearance rate of PpIX in WiDr cells was faster after application of a low concentration of ALA (0.1 mM) than after application of high concentration of ALA (1 mM). PpIX was cleared faster from cells which initially were seeded at low densities than cells seeded at higher densities. The presence of the iron chelator deferoxamine reduced the clearance rate of PpIX, while the presence of ferrous sulfate acted oppositely. The decay rate of PpIX in WiDr cells was faster at higher temperature than at lower. The ferrochelatase activity at pH 7.2 was significantly greater than that at pH 6.7. ALA concentration, application time, cell density, temperature, pH, intracellular iron content, intracellular amount and localization of PpIX are factors controlling PpIX clearance.

Citation Download Citation

Asta Juzeniene, Miron Kaliszewski, Andrzej Bugaj, and Johan Moan "Clearance of protoporphyrin IX induced by 5-aminolevulinic acid from WiDr human colon carcinoma cells", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73802Q (13 July 2009); https://doi.org/10.1117/12.822944

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