High-resolution imaging of a corneal incision by second- and third-harmonic generation microscopy

L. Jay; C. Dion; A. Brocas; K. Singh; J.-C. Kieffer; I. Brunette; T. Ozaki

doi:10.1117/12.842078

26 February 2010 High-resolution imaging of a corneal incision by second- and third-harmonic generation microscopy

L. Jay, C. Dion, A. Brocas, K. Singh, J.-C. Kieffer, I. Brunette, T. Ozaki

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692T (2010) https://doi.org/10.1117/12.842078
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We demonstrate high spatial resolution imaging of a stromal cut in the ex-vivo pig cornea, using second- and third-harmonic generation microscopy. From these images, we see in detail how the cut affects the corneal layers. In the beginning of the cut, the anterior layers, in which the blade is passing through, are disorganized, which could explain the shadows observed on the images. In the stroma, the cut can be imaged by third harmonic microscopy, probably due to the χ³ contrast. Although the current results were obtained from the healthy ex-vivo cornea, it already allows one to understand the effects of the cut on the tissue characteristics (such as scattering).

Citation Download Citation

L. Jay, C. Dion, A. Brocas, K. Singh, J.-C. Kieffer, I. Brunette, and T. Ozaki "High-resolution imaging of a corneal incision by second- and third-harmonic generation microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692T (26 February 2010); https://doi.org/10.1117/12.842078

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