Off-axis phase-shifting holographic interferometry for the 3D localization of cellular transmembrane receptors tagged with gold nanomarkers

Fadwa Joud; Nilanthi Warnasooriya; Philippe Bun; Gilles Tessier; Maïte Coppey-Moisan; Michael Atlan; Pierre Desbiolles; Marie Abboud; Michel Gross

doi:10.1117/12.840496

16 February 2010 Off-axis phase-shifting holographic interferometry for the 3D localization of cellular transmembrane receptors tagged with gold nanomarkers

Fadwa Joud, Nilanthi Warnasooriya, Philippe Bun, Gilles Tessier, Maïte Coppey-Moisan, Michael Atlan, Pierre Desbiolles, Marie Abboud, Michel Gross

Author Affiliations +

Proceedings Volume 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II; 757610 (2010) https://doi.org/10.1117/12.840496
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In this communication, we show that off-axis digital holography combined to phase-shifting acquisition of holograms is an effective microscopic tool to fully localize, in three dimensions, transmembrane receptors of living cells tagged with Gold nanocolloids. Gold nanoparticles, known for their interesting optical properties as well as for their noncytotoxicity are used here as biomarkers to target the cellular receptors.

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Fadwa Joud, Nilanthi Warnasooriya, Philippe Bun, Gilles Tessier, Maïte Coppey-Moisan, Michael Atlan, Pierre Desbiolles, Marie Abboud, and Michel Gross "Off-axis phase-shifting holographic interferometry for the 3D localization of cellular transmembrane receptors tagged with gold nanomarkers", Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 757610 (16 February 2010); https://doi.org/10.1117/12.840496

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