A porous silicon based microarray for label-free optical detection of DNA hybridization

Ilaria Rea; Giuseppe Coppola; Mariano Gioffrè; Edoardo De Tommasi; Ivo Rendina; Annalisa Lamberti; Luca De Stefano

doi:10.1117/12.848471

16 February 2010 A porous silicon based microarray for label-free optical detection of DNA hybridization

Ilaria Rea, Giuseppe Coppola, Mariano Gioffrè, Edoardo De Tommasi, Ivo Rendina, Annalisa Lamberti, Luca De Stefano

Proceedings Volume 7606, Silicon Photonics V; 76060E (2010) https://doi.org/10.1117/12.848471
Event: SPIE OPTO, 2010, San Francisco, California, United States

Abstract

In this work, we have fabricated a porous silicon (PSi) Bragg reflectors microarray using a proper technological process based on photolithography and electrochemical anodization of silicon. Each element of the array is characterized by a diameter of 200 μm. The PSi structures have been used as platform to immobilize label-free DNA probe and a simple optical method has been employed to investigate the interaction between probe-DNA and its complementary target. In order to confirm the specificity of the DNA hybridization, we have also verified that the reaction of probe-DNA with non-complementary DNA did not occur.

Citation Download Citation

Ilaria Rea, Giuseppe Coppola, Mariano Gioffrè, Edoardo De Tommasi, Ivo Rendina, Annalisa Lamberti, and Luca De Stefano "A porous silicon based microarray for label-free optical detection of DNA hybridization", Proc. SPIE 7606, Silicon Photonics V, 76060E (16 February 2010); https://doi.org/10.1117/12.848471

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